Biodegradation of Inosine and Guanosine by Bacillus paranthracis YD01

Both inosine and guanosine are precursors of uric acid that may cause the diseases hyperuricemia gout in humans. Here, a promising bacterial strain for efficiently biodegrading both was successfully isolated from healthy human intestine identified as Bacillus paranthracis YD01 with 16S rRNA analysis. An initial amount 49.6 mg·L−1 or 49.9 completely removed by within 12 h, which showed had strong ability to biodegrade guanosine. Furthermore, 49.2 49.5 totally catalyzed intracellular crude enzymes 6 49.7 biodegraded extracellular 9 h. Illumina Hiseq sequencing database gene annotation were used elucidate genomic characteristics B. YD01. Purine nucleoside phosphorylase, encoded 1785, 3933, 4403, found KEEG database, played crucial role biodegradation The results this study provide valuable insights into mechanisms using